Quantification of Pseudomonas protegens FD6 and Bacillus subtilis NCD-2 in soil and the wheat rhizosphere and suppression of root pathogenic Rhizoctonia solani AG-8

Bacillus subtilis strain NCD-2 and Pseudomonas protegens FD6 produce a range of antimicrobial metabolites known to be effective against soil-borne plant pathogens. Quantitative qPCR assays were designed from sequence characterized amplified fragment length polymorphisms (AFLP) diagnostic for each inoculant genotype. The minimum detection thresholds (MDT) ranged between 5.2 × 103 (log 3.71) 1.5 106 6.18) genome (cell) equivalents g?1 soil or wheat root tissue independent type. Wheat rhizosphere populations strains significantly declined during the first 14 days postinoculation then remained stable next days. Colonization roots, bulk (non-rhizosphere) by was greater than that NCD-2. Strains decreased in vitro growth pathogenic Rhizoctonia solani AG-8, Pythium irregulare Gaeumannomyces graminis var. tritici, fungal antibiosis colonization abundance R. AG-8 3- 13-fold respectively, pathogen suppression increasing (21%–29%) foliar biomass seedlings levels not different non-diseased control. developed this study used monitor P. B. their suppressive efficacies wheat. These can applied dynamics these inoculants suppressing rot other crop diseases.